hrvatski jezikClear Cookie - decide language by browser settings

MALDI-TOF MS profiling of piscine Acinetobacter spp. from wastewater-related waters

Topić Popović, Natalija; Kazazić, Snježana; Čož- Rakovac, Rozelinda (2022) MALDI-TOF MS profiling of piscine Acinetobacter spp. from wastewater-related waters. Veterinarski glasnik, 00 . pp. 1-8. ISSN 0350-2457

PDF - Published Version - article
Available under License Creative Commons Attribution.

Download (864kB) | Preview


Acinetobacter species are common inhabitants of freshwater and marine ecosystems with a capacity to induce disease in affected fish. To facilitate their rapid and reliable identification, matrix- assisted laser desorption/ionization time-of- flight mass spectrometry (MALDI-TOF MS), was applied to Acinetobacter from fish. The aims of the study were, thus, to identify and profile the Acinetobacter species from tissues of fish living in a vulnerable environment impacted by wastewaters, and to assess the potential of MALDI- TOF MS as a method for discriminating these acinetobacters. Fish were sampled from waterways impacted by the activity of a wastewater treatment plant. Samples of gills, spleen, kidney and liver were streaked onto general-purpose media to purity. The profiling and identification of acinetobacters was conducted with MALDITOF MS, with the samples prepared by ethanol/formic acid extraction. The identified acinetobacters were retrieved from gills (68.96 %), kidney (13.79 %), liver (10.34 %), and spleen (6.89 %). The Acinetobacter species isolated from all tested fish tissues were A. johnsonii (79.31 %), A. pittii (10.34 %), A. tandoii (3.44 %), A. guilouiae (3.44 %), and A. gerneri (3.44 %). Highly probable and probable species identifications were obtained for 48.27 % of all acinetobacters tested, indicating fully reliable identification. MALDI‐TOF MS gave excellent identification and profiling results for piscine Acinetobacter species from the wastewater-affected waterways. It is a recommendable technique for future Acinetobacter species discrimination, as accurate and rapid identification of these bacteria is critical in both environmental pollution management and human/veterinary clinical diagnostics.

Item Type: Article
Uncontrolled Keywords: Acinetobacter, fish, MALDI-TOF MS, wastewater
Subjects: NATURAL SCIENCES > Biology
Divisions: Division of Materials Chemistry
Division of Physical Chemistry
Project titleProject leaderProject codeProject type
Bioprospecting Jadranskog moraČož-Rakovac, Rozelinda; Dragović-Uzelac, Verica; Šantek, Božidar; Jokić, Stela; Jerković, Igor; Kraljević Pavelić, SandraKK.
Depositing User: Natalija Topić Popović
Date Deposited: 11 Jul 2022 07:27
DOI: 10.2298/VETGL211201005T

Actions (login required)

View Item View Item


Downloads per month over past year

Increase Font
Decrease Font
Dyslexic Font