Characterization and evolution of centromere in nematodes of the genus Meloidogyne

Despot-Slade, Evelin (2022) Characterization and evolution of centromere in nematodes of the genus Meloidogyne. Doctoral thesis, Sveučilište Josipa Jurja Strossmayera u Osijeku, Sveučilište u Dubrovniku, Institut Ruđer Bošković, Sveučilište Josipa Jurja Strossmayera u Osijeku, Sveučilište u Dubrovniku, Institut Ruđer Bošković.

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Abstract

Centromeres are chromosomal regions responsible for the faithful segregation of chromosomes. In contrast to conserved function, the centromere protein (CenH3) and associated repetitive DNA evolve rapidly. However, our study revealed preservation of the αCenH3 variant along with conserved 19-bp box sequence in rapidly evolved centromeric DNA in asexual Meloidoygne nematodes. A similar approach was applied to the beetle Tribolium castaneum and showed a metapolycentric centromere organization with extended centromere regions. Long-range Nanopore sequencing is optimized for further study of centromeric arrays in the genomes of Meloidogyne and Tribolium species.

Item Type:	Thesis (Doctoral thesis)
Uncontrolled Keywords:	centromere ; CenH3 ; centromeric DNA ; nematode ; Meloidogyne ; metapolycentromere ; beetle ; Tribolium castaneum ; satellite DNA ; Nanopore sequencing
Subjects:	NATURAL SCIENCES NATURAL SCIENCES > Biology NATURAL SCIENCES > Biology > Genetics, Evolution and Phylogenetics
Divisions:	Division of Molecular Biology
Projects:	Project title Project leader Project code Project type Centromerna genomika beskralježnjaka Plohl, Miroslav IP-2014-09-3183 HRZZ (Epi)genomika i transkriptomika eukromatinskih satelitnih DNA u embriogenezi i razvoju Meštrović Radan, Nevenka IP-2019-04-4910 HRZZ
Depositing User:	Evelin Despot-Slade
Date Deposited:	15 Jun 2022 12:11
URI:	http://fulir.irb.hr/id/eprint/7386

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