hrvatski jezikClear Cookie - decide language by browser settings

Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features

Velle, Katrina B.; Kennard, Andrew S.; Trupinić, Monika; Ivec, Arian; Swafford, Andrew J.M.; Nolton, Emily; Rice, Luke M.; Tolić, Iva M.; Fritz-Laylin, Lillian K.; Wadsworth, Patricia (2022) Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features. Current Biology, 32 (6). 1247-1261.e6. ISSN 09609822

PDF - Published Version - article
Available under License Creative Commons Attribution.

Download (13MB) | Preview


Naegleria gruberi is a unicellular eukaryote whose evolutionary distance from animals and fungi has made it useful for developing hypotheses about the last common eukaryotic ancestor. Naegleria amoebae lack a cytoplasmic microtubule cytoskeleton and assemble microtubules only during mitosis and thus represent a unique system for studying the evolution and functional specificity of mitotic tubulins and the spindles they assemble. Previous studies show that Naegleria amoebae express a divergent α-tubulin during mitosis, and we now show that Naegleria amoebae express a second mitotic α- and two mitotic β-tubulins. The mitotic tubulins are evolutionarily divergent relative to typical α- and β-tubulins and contain residues that suggest distinct microtubule properties. These distinct residues are conserved in mitotic tubulin homologs of the “brain-eating amoeba” Naegleria fowleri, making them potential drug targets. Using quantitative light microscopy, we find that Naegleria’s mitotic spindle is a distinctive barrel-like structure built from a ring of microtubule bundles. Similar to those of other species, Naegleria’s spindle is twisted, and its length increases during mitosis, suggesting that these aspects of mitosis are ancestral features. Because bundle numbers change during metaphase, we hypothesize that the initial bundles represent kinetochore fibers and secondary bundles function as bridging fibers.

Item Type: Article
Uncontrolled Keywords: evolutionary cell biology; spindle; cytoskeleton; mitosis; microtubules; Naegleria; protist; tubulin
Subjects: NATURAL SCIENCES > Physics > Biophysics and Medical Physics
NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology
Divisions: Division of Molecular Biology
Project titleProject leaderProject codeProject type
Molecular origins of aneuploidies in healthy and diseased human tissues-ANEUPLOIDYIva Tolić; Nenad Pavin855158EK
Ravnoteža sila i momenta sila u diobenom vretenu-SpindleForceBalanceNenad PavinIP-2019-04-5967HRZZ
Provedba vrhunskih istraživanja u sklopu Znanstvenog centra izvrsnosti za kvantne i kompleksne sustave te reprezentacije Liejevih algebri-QuantiXLieHrvoje Buljan; Pavle PandžićKK.
Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini-IPStedIva TolićKK.
Depositing User: Maša Sabo
Date Deposited: 14 Apr 2022 12:03
DOI: 10.1016/j.cub.2022.01.034

Actions (login required)

View Item View Item


Downloads per month over past year

Increase Font
Decrease Font
Dyslexic Font