DNA barcoding of the horsefly fauna (Diptera, Tabanidae) of Croatia with notes on the morphology and taxonomy of selected species from Chrysopsinae and Tabaninae

Krčmar, Stjepan; Kučinić, Mladen; Pezzi, Marco; Bruvo Mađarić, Branka (2022) DNA barcoding of the horsefly fauna (Diptera, Tabanidae) of Croatia with notes on the morphology and taxonomy of selected species from Chrysopsinae and Tabaninae. ZooKeys (1087). pp. 141-161. ISSN 1313-2989

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Official URL: https://zookeys.pensoft.net/article/78707/

Abstract

In the Croatian fauna, horseflies (Tabanidae) are represented by 78 species belonging to two subfamilies, five tribes, and 10 genera. Identification of these species is based on morphological characteristics. In this study, 43 species of horseflies were analyzed. The highest number of species (19) belongs to the genus Tabanus, followed by the genera Hybomitra with seven species, Haematopota with six species, Chrysops with four species, Atylotus and Philipomyia with two species each, and the genera Silvius, Dasyrhamphis, and Heptatoma with one species each. The standard DNA barcoding region of the mitochondrial cytochrome c oxidase gene, subunit I (COI), was sequenced and compared to the Barcode of Life Database (BOLD). Our analyses confirmed our morphological identifications and added 16 new Barcode Index Numbers (BINs) for Tabanidae to BOLD. Potential problems in the systematics and taxonomy of this family are highlighted.

Item Type:

Article

Uncontrolled Keywords:

Barcode Index Number (BIN) ; cytochrome c oxidase gene subunit I (COI), Molecular Operational Taxonomic Unit (MOTU) ; species delimitation ; vector species

Subjects:

NATURAL SCIENCES > Biology

Divisions:

Division of Molecular Biology

Projects:

Project title	Project leader	Project code	Project type
DNA barkodiranje bioraznolikosti hrvatske faune	Kučinić, Mladen	IP-2016-06-9988	HRZZ
Alfa satelitska DNA u evoluciji mreža genske modulacije	Ugarković, Đurđica	IP-2019-04-6915	HRZZ

Depositing User:

Sofija Konjević

Date Deposited:

10 Mar 2022 13:01

URI:

http://fulir.irb.hr/id/eprint/7061

DOI:

10.3897/zookeys.1087.78707