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Flavylium dye as pH tunable fluorescent and CD probe for double-stranded DNA and RNA

Crnolatac, Ivo; Giestas, Letícia; Horvat, Gordan; Parola, A. Jorge; Piantanida, Ivo (2020) Flavylium dye as pH tunable fluorescent and CD probe for double-stranded DNA and RNA. Chemosensors, 8 (4). ISSN 2227-9040

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Abstract

The interaction of 4′-(N, N-dimethylamino)-6-hydroxyflavylium cation with ds-DNA/RNA was studied by UV/Vis, circular dichroism (CD) spectrophotometry, and also steady-state and time-resolved emission spectroscopies at neutral and weakly acidic conditions. At pH 5, the studied molecule, in its flavylium cationic form, showed considerable binding affinities (5 < logKs < 6) for all ds-DNA/RNA, contrary to chalcones forms (dominant at pH 7), which did not show binding to polynucleotides. Flavylium cation intercalated into ds-DNAs at variance to dominant groove aggregation within ds-RNA, which was reported by RNA-specific bisignate induced CD spectrum (ICD) bands. The intrinsically negligible fluorescence of flavylium was strongly increased upon the addition of DNA or RNA, whereby both the fluorescence intensity and emission lifetimes of complexes differed considerably: the strongest emission increase was observed for AU-RNA (detection limit estimated to 10 nM) followed by AT-DNAs and the much weaker effect of GC-DNAs. Both fluorescence sensitivity on the ds-DNA/RNA secondary structure and sequence-selective ICD bands make the flavylium–chalcones system an intriguing pH-switchable new probe for distinguishing between various polynucleotide sequences.

Item Type: Article
Uncontrolled Keywords: flavylium cation ; chalcones ; pH control ; DNA/RNA binding ; fluorescence, circular dichroism
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Division of Organic Chemistry and Biochemistry
Projects:
Project titleProject leaderProject codeProject type
Višekromoforne probe za prepoznavanje pojedinih struktura DNA, RNA i proteinaPiantanida, IvoIP-2018-01-5475HRZZ
Depositing User: Ivo Crnolatac
Date Deposited: 07 Mar 2022 10:47
URI: http://fulir.irb.hr/id/eprint/7044
DOI: 10.3390/chemosensors8040129

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