Puljko, Ana; Milaković, Milena; Krizanović, Stela; Kosić-Vukšić, Josipa; Babić, Ivana; Petrić, Ines; Maravić, Ana; Jelić, Marko; Udiković-Kolić, Nikolina (2022) Prevalence of enteric opportunistic pathogens and extended-spectrum cephalosporin- and carbapenem-resistant coliforms and genes in wastewater from municipal wastewater treatment plants in Croatia. Journal of Hazardous Materials, 427 . ISSN 0304-3894
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Abstract
Extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing Enterobacterales are a critical global health problem and wastewater treatment plants (WWTPs) can promote their spread into the environment; yet their efficacy is not well characterized. Here, we have used conventional culturing to monitor coliform bacteria and quantitative PCR to monitor 2 ESBL and 5 carbapenemase (CP) genes and 4 enteric opportunistic pathogens (EOPs) in the influent and effluent of 7 Croatian WWTPs in two seasons. In general, levels of total, cefotaxime and carbapenem-resistant coliforms were significantly reduced but not eliminated by conventional treatment in most WWTPs. Most WWTPs efficiently removed EOPs such as K. pneumoniae and A. baumannii, while E. coli and Enterococcus spp. were reduced but still present in relatively high concentrations in the effluent. ESBL genes (blaTEM and blaCTX-M-32) were only slightly reduced or enriched after treatment. CP genes, blaKPC-3, blaNDM and blaOXA-48-like, were sporadically detected, while blaIMP and blaVIM were frequently enriched during treatment and correlated with plant size, number or size of hospitals in the catchment area, and COD effluent concentration. Our results suggest that improvements in wastewater treatment technologies are needed to minimize the risk of environmental contamination with top priority EOPs and ARGs and the resulting public health.
| Item Type: | Article | ||||||||
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| Uncontrolled Keywords: | Wastewater treatment; ESBL; Carbapenemase; Antibiotic resistance genes; Enteric opportunistic pathogens | ||||||||
| Subjects: | BIOTECHNICAL SCIENCES > Biotechnology BIOTECHNICAL SCIENCES > Biotechnology > Molecular Biotechnology BIOTECHNICAL SCIENCES > Biotechnology > Waste Management BIOTECHNICAL SCIENCES > Interdisciplinary Technical Sciences |
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| Divisions: | Division for Marine and Enviromental Research | ||||||||
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| Depositing User: | Ana Puljko | ||||||||
| Date Deposited: | 06 Apr 2023 14:14 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/6949 | ||||||||
| DOI: | 10.1016/j.jhazmat.2021.128155 |
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