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Chemical Evaluation, Antioxidant, Antiproliferative, Anti-Inflammatory and Antibacterial Activities of Organic Extract and Semi-Purified Fractions of the Adriatic Sea Fan, Eunicella cavolini

Matulja, Dario; Grbčić, Petra; Bojanić, Krunoslav; Topić Popović, Natalija; Čož-Rakovac, Rozelindra; Laclef, Sylvain; Šmuc, Tomislav; Jović, Ozren; Marković, Dean; Pavelić, Sandra Kraljević (2021) Chemical Evaluation, Antioxidant, Antiproliferative, Anti-Inflammatory and Antibacterial Activities of Organic Extract and Semi-Purified Fractions of the Adriatic Sea Fan, Eunicella cavolini. Molecules, 26 (19). ISSN 1420-3049

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Abstract

Due to sedentary lifestyle and harsh environmental conditions, gorgonian coral extracts are recognized as a rich source of novel compounds with various biological activities, of interest to the pharmaceutical and cosmetic industries. The presented study aimed to perform chemical screening of organic extracts and semi-purified fractions obtained from the common Adriatic gorgonian, sea fan, Eunicella cavolini (Koch, 1887) and explore its abilities to exert different biological effects in vitro. Qualitative chemical evaluation revealed the presence of several classes of secondary metabolites extended with mass spectrometry analysis and tentative dereplication by using Global Natural Product Social Molecular Networking online platform (GNPS). Furthermore, fractions F4 and F3 showed the highest phenolic (3.28 ± 0.04 mg GAE/g sample) and carotene (23.11 ± 2.48 mg β-CA/g sample) content, respectively. The fraction F3 inhibited 50% of DPPH (2, 2-diphenyl-1-picryl- hydrazyl-hydrate) and ABTS (2, 2′-azino-bis (3- ethylbenzthiazolin-6-yl) sulfonic acid) radicals at the concentrations of 767.09 ± 11.57 and 157.16 ± 10.83 µg/mL, respectively. The highest anti-inflammatory potential was exhibited by F2 (IC50 = 198.70 ± 28.77 µg/mL) regarding the inhibition of albumin denaturation and F1 (IC50 = 254.49 ± 49.17 µg/mL) in terms of soybean lipoxygenase inhibition. In addition, the most pronounced antiproliferative effects were observed for all samples (IC50 ranging from 0.82 ± 0.14–231.18 ± 46.13 µg/mL) against several carcinoma cell lines, but also towards non-transformed human fibroblasts pointing to a generally cytotoxic effect. In addition, the antibacterial activity was tested by broth microdilution assay against three human pathogenic bacteria: Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The latter was the most affected by fractions F2 and F3. Finally, further purification, isolation and characterization of pure compounds from the most active fractions are under investigation.

Item Type: Article
Uncontrolled Keywords: biological activities ; biological screening ; Eunicella cavolini ; gorgonian corals ; organic extracts
Subjects: NATURAL SCIENCES > Chemistry
INTERDISCIPLINARY AREAS OF KNOWLEDGE > Biotechnology in Biomedicine (natural science, biomedicine and healthcare, bioethics area
Divisions: Division of Electronics
Division of Materials Chemistry
Projects:
Project titleProject leaderProject codeProject type
Bioprospecting Jadranskog moraČož-Rakovac, Rozelinda; Dragović-Uzelac, Verica; Šantek, Božidar; Jokić, Stela; Jerković, Igor; Kraljević Pavelić, SandraKK.01.1.1.01.0002EK
Razvoj istraživačke infrastrukture na kampusu Sveučilišta u RijeciOžanić, NevenkaRC.2.2.06-0001EK
In-silico potpomognut dizajn supstrata za hvatanje CO2 u katalitičkoj formaciji karbonata i karbamataMarković, DeanIP-2019-04-8846HRZZ
Depositing User: Natalija Topić Popović
Date Deposited: 15 Nov 2021 13:58
Last Modified: 19 Nov 2021 13:45
URI: http://fulir.irb.hr/id/eprint/6636
DOI: 10.3390/molecules26195751

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