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Sample preparation and culture conditions effects on MALDI-TOF MS identification of bacteria: A review

Topić Popović, Natalija; Kazazić, Snježana; Bojanić, Krunoslav; Strunjak-Perović, Ivančica; Čož-Rakovac, Rozelindra (2021) Sample preparation and culture conditions effects on MALDI-TOF MS identification of bacteria: A review. Mass spectrometry reviews . ISSN 0277-7037

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Abstract

Matrix-assisted laser desorption/ionization time- of-flight mass spectrometry(MALDI-TOF MS) is an excellent tool for bacterial identification. It allows high throughput, sensitive and specific applications in clinical diagnostics and environ- mental research. Currently, there is no optimal standardized protocol for sample preparation and culture conditions to profile bacteria. The performance of MALDITOF MS is affected by several variables, such as sample preparation, culture media and culture conditions, incubation time/growth stage, incubation temperature, high salt content, blood in the culture media, and others. This review thus aims to clarify why a uniformed protocol is not plausible, to assess the effects these factors have onMALDITOF MS identification score, and discuss possible optimizations for its methodology, in relation to specific bacterial representatives and strain requirements.

Item Type: Article
Uncontrolled Keywords: Bacteria ; Culture conditions ; Diagnostics ; MALDI-TOF MS ; Sample preparation
Subjects: NATURAL SCIENCES > Biology
Divisions: Division of Materials Chemistry
Division of Materials Physics
Projects:
Project titleProject leaderProject codeProject type
Znanstveni centar izvrsnosti za bioprospecting mora-BioProCroRozelinda Čož-Rakovac; Marin RojeN/AMZOS
Depositing User: Natalija Topić Popović
Date Deposited: 15 Nov 2021 13:50
Last Modified: 16 Nov 2021 12:47
URI: http://fulir.irb.hr/id/eprint/6631
DOI: 10.1002/mas.21739

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