Chilling and Freezing Temperature Stress Differently Influence Glucosinolates Content in Brassica oleracea var. acephala

Ljubej, Valentina; Radojčić Redovniković, Ivana; Salopek Sondi, Branka; Smolko, Ana; Roje, Sanja; Šamec, Dunja (2021) Chilling and Freezing Temperature Stress Differently Influence Glucosinolates Content in Brassica oleracea var. acephala. Plants, 10 (7). ISSN 2223-7747

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Official URL: https://www.mdpi.com/2223-7747/10/7/1305

Abstract

Brassica oleracea var. acephala is known to have a strong tolerance to low temperatures, but the protective mechanisms enabling this tolerance are unknown. Simultaneously, this species is rich in health-promoting compounds such as polyphenols, carotenoids, and glucosinolates. We hypothesize that these metabolites play an important role in the ability to adapt to low temperature stress. To test this hypothesis, we exposed plants to chilling (8 °C) and additional freezing (−8 °C) temperatures under controlled laboratory conditions and determined the levels of proline, chlorophylls, carotenoids, polyphenols, and glucosinolates. Compared with that of the control (21 °C), the chilling and freezing temperatures increased the contents of proline, phenolic acids, and flavonoids. Detailed analysis of individual glucosinolates showed that chilling increased the total amount of aliphatic glucosinolates, while freezing increased the total amount of indolic glucosinolates, including the most abundant indolic glucosinolate glucobrassicin. Our data suggest that glucosinolates are involved in protection against low temperature stress. Individual glucosinolate species are likely to be involved in different protective mechanisms because they show different accumulation trends at chilling and freezing temperatures.

Item Type:

Article

Uncontrolled Keywords:

Brassica oleracea var. acephala ; low temperature stress ; glucosinolates ; polyphenols ; abiotic stress

Subjects:

NATURAL SCIENCES > Biology

Divisions:

Division of Molecular Biology

Projects:

Project title	Project leader	Project code	Project type
Metabolički i transkriptomski odgovor raštike na stres izazvan niskim temperaturama	Šamec, Dunja	12/17	HRZZ
Agrobioraznolikost - osnova za prilagodbu i ublažavanje posljedica klimatskih promjena u poljoprivredi	Goreta Ban, Smiljana; Salopek Sondi, Branka; Bauer, Nataša; Jasprica, Nenad; Kereša, Snježana	KK.05.1.1.02.0005	EK

Depositing User:

Dunja Šamec

Date Deposited:

29 Jun 2021 11:49

URI:

http://fulir.irb.hr/id/eprint/6473

DOI:

10.3390/plants10071305