Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires

Babić, Sanja; Čižmek, Lara; Maršavelski, Aleksandra; Malev, Olga; Pflieger, Maryline; Strunjak-Perović, Ivančica; Topić Popović, Natalija; Čož-Rakovac, Rozelindra; Trebše, Polonca (2021) Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires. Scientific reports, 11 . ISSN 2045-2322

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Official URL: https://www.nature.com/articles/s41598-021-81789-1

Abstract

Amazonian wildfires in 2019 have raised awareness about rainforest burning due to increased emissions of particulate matter and carbon. In the context of these emissions, by-products of lignin thermal degradation (i.e. methoxyphenols) are often neglected. Methoxyphenols entering the atmosphere may form intermediates with currently unknown reaction mechanisms and toxicity. This study for the first time provides a comprehensive insight into the impact of lignin degradation products [guaiacol, catechol], and their nitrated intermediates [4-nitrocatechol, 4, 6- dinitroguaiacol, 5-nitroguaiacol] on zebrafish Danio rerio. Results revealed 4-nitrocatechol and catechol as the most toxic, followed by 4, 6DNG > 5NG > GUA. The whole-organism bioassay integrated with molecular modeling emphasized the potential of methoxyphenols to inhibit tyrosinase, lipoxygenase, and carbonic anhydrase, consequently altering embryonic development (i.e. affected sensorial, skeletal, and physiological parameters, pigmentation formation failure, and non-hatching of larvae). The whole-organism bioassay integrated with in silico approach confirmed the harmful effects of lignin degradation products and their intermediates on aquatic organisms, emphasizing the need for their evaluation within ecotoxicity studies focused on aquatic compartments.

Item Type:

Article

Uncontrolled Keywords:

biomass burning ; methoxyphenols ; nitrated intermediates ; atmospheric pollution ; zebrafish embryotoxicity

Subjects:

NATURAL SCIENCES > Biology

Divisions:

Division of Materials Chemistry

Projects:

Project title	Project leader	Project code	Project type
Bioprospecting Jadranskog mora	Čož-Rakovac, Rozelinda; Dragović-Uzelac, Verica; Jokić, Stela; Jerković, Igor; Kraljević Pavelić, Sandra	KK.01.1.1.01.0002	EK

Depositing User:

Sanja Babić

Date Deposited:

11 Feb 2021 11:57

Last Modified:

10 Aug 2021 13:07

URI:

http://fulir.irb.hr/id/eprint/6327

DOI:

10.1038/s41598-021-81789-1