hrvatski jezikClear Cookie - decide language by browser settings

ATP synthase modulation leads to an increase of spare respiratory capacity in HPV associated cancers

Kirschberg, Matthias; Heuser, Sandra; Marcuzzi, Gian Paolo; Hufbauer, Martin; Seeger, Jens Michael; Đukić, Anamaria; Tomaić, Vjekoslav; Majewski, Slawomir; Wagner, Steffen; Wittekindt, Claus; Würdemann, Nora; Klussmann, Jens Peter; Quaas, Alexander; Kashkar, Hamid; Akgül, Baki (2020) ATP synthase modulation leads to an increase of spare respiratory capacity in HPV associated cancers. Scientific reports, 10 (1). ISSN 2045-2322

PDF - Published Version - article
Available under License Creative Commons Attribution.

Download (3MB) | Preview


Mucosal and skin cancers are associated with infections by human papillomaviruses (HPV). The manner how viral oncoproteins hijack the host cell metabolism to meet their own energy demands and how this may contribute to tumorigenesis is poorly understood. We now show that the HPV oncoprotein E7 of HPV8, HPV11 and HPV16 directly interact with the beta subunit of the mitochondrial ATP-synthase (ATP5B), which may therefore represent a conserved feature across different HPV genera. By measuring both glycolytic and mitochondrial activity we observed that the association of E7 with ATP5B was accompanied by reduction of glycolytic activity. Interestingly, there was a drastic increase in spare mitochondrial respiratory capacity in HPV8-E7 and an even more profound increase in HPV16-E7 expressing cells. In addition, we could show that ATP5B levels were unchanged in betaHPV positive skin cancers. However, comparing HPV-positive and HPV-negative oropharyngeal squamous cell carcinomas (OPSCC) we noticed that, while ATP5B expression levels did not correlate with patient overall survival in HPV-negative OPSCC, there was a strong correlation within the HPV16-positive OPSCC patient group. These novel findings provide evidence that HPV targets the host cell energy metabolism important for viral life cycle and HPV-mediated tumorigenesis.

Item Type: Article
Uncontrolled Keywords: human papillomaviruses (HPV), cell metabolism, mitochondrial ATP-synthase (ATP5B), HPV-mediated tumorigenesis
Subjects: BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences
Divisions: Division of Molecular Medicine
Project titleProject leaderProject codeProject type
Rasvjetljivanje onkogenih funkcija E6/E7 HPV-a na različitim anatomskim mjestima-HPVHNCVjekoslav TomaićIP-2016-06-2246HRZZ
Depositing User: Vjekoslav Tomaić
Date Deposited: 08 Dec 2020 14:35
DOI: 10.1038/s41598-020-74311-6

Actions (login required)

View Item View Item


Downloads per month over past year

Increase Font
Decrease Font
Dyslexic Font