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Effect of Enzymatic, Ultrasound, and Reflux Extraction Pretreatments on the Chemical Composition of Essential Oils

Miljanović, Anđela; Bielen, Ana; Grbin, Dorotea; Marijanović, Zvonimir; Andlar, Martina; Rezić, Tonči; Roca, Sunčica; Jerković, Igor; Vikić-Topić, Dražen; Dent, Maja (2020) Effect of Enzymatic, Ultrasound, and Reflux Extraction Pretreatments on the Chemical Composition of Essential Oils. Molecules, 25 (20). ISSN 1420-3049

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The effect of different hydrodistillation pretreatments, namely, reflux extraction, reflux extraction with the addition of cell wall- degrading enzymes, and ultrasound, on the yield and chemical composition of essential oils of sage, bay laurel, and rosemary was examined. All pretreatments improved essential oil yield compared to no-pretreatment control (40–64% yield increase), while the oil quality remained mostly unchanged (as shown by statistical analysis of GC-MS results). However, enzyme-assisted reflux extraction pretreatment did not significantly outperform reflux extraction (no-enzyme control), suggesting that the observed yield increase was mostly a consequence of reflux extraction and enzymatic activity had only a minute effect. Thus, we show that ultrasound and reflux extraction pretreatments are beneficial in the production of essential oils of selected Mediterranean plants, but the application of enzymes has to be carefully re-evaluated.

Item Type: Article
Uncontrolled Keywords: Clevenger hydrodistillation ; Salvia officinalis L. ; Rosmarinus officinalis L. ; Laurus nobilis L. ; cell wall-degrading enzymes ; ultrasound pretreatment ; reflux extraction pretreatment ; GC-MS analysis
Subjects: NATURAL SCIENCES > Biology
Divisions: NMR Center
Project titleProject leaderProject codeProject type
Interakcije slatkovodnih patogenih oomiceta i okoliša-InteractOomycAna BielenUIP-2017-05-6267HRZZ
Depositing User: Sunčica Roca
Date Deposited: 10 Nov 2020 11:50
DOI: 10.3390/molecules25204818

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