The effect of different pollutants exposure on the pigment content of pigmented macrophage aggregates in the spleen of Vardar chub (Squalius vardarensis Karaman, 1928)

Ivanova, Lozenka; Rebok, Katerina; Jordanova, Maja; Dragun, Zrinka; Kostov, Vasil; Ramani, Sheriban; Valić, Damir; Krasnići, Nesrete; Filipović Marijić, Vlatka; Kapetanović, Damir (2020) The effect of different pollutants exposure on the pigment content of pigmented macrophage aggregates in the spleen of Vardar chub (Squalius vardarensis Karaman, 1928). Microscopy Research and Technique, 83 . pp. 1141-1152. ISSN 1059-910X

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Abstract

Pigmented macrophage aggregates (MAs) are known to change under influence of various factors, such as aging, season, starvation and/or pollution. In this study, changes in the pigment content of the MAs in the spleen of Vardar chub (Squalius vardarensis, Karaman) (n=129) collected in spring and autumn, from three rivers with different pollution impact was examined: Zletovska River (metals), Kriva River (metals and municipal wastewater), Bregalnica River (municipal wastewater). Collected data revealed increased relative volume and number of MAs containing hemosiderin under the influence of metals, significant in autumn (p<0.05). In chub exposed to metals combined with municipal wastewater, significant increase of lipochrome accumulation in MAs in autumn, melanin in MAs in fish captured in the spring season, and number of splenic MAs containing combination of melanin and lipochrome was noted. Volumes and number of MAs containing combination of hemosiderin and lipochrome increased in spleen of fish captured in autumn from both Zletovska River and Kriva River, most likely due to the contribution of hemosiderin and lipochrome, respectively. Values measured for the various pigments in splenic MAs in fish captured from Bregalnica River, were overall closer to the values measured for fish captured from Kriva River. Notably, melanin and lipochrome are more likely to be found in fish from waters influenced by municipal wastewater (organic pollution) and hemosiderin in fish spleen from water influenced by mining activity (heavy metals pollution).

Item Type:	Article
Additional Information:	The authors are thankful to all those having contributed to the study. The authors are especially thankful to Prof Zlatko Levkov for the use of equipment, the light microscope, and digital camera for making of microphotographs. This study has been financially supported by the Ministry of Science, Education and Sport of the Republic of Croatia (project Nos. 098-0982934-2721 and 098-0982934-2752) and by the Ministry of Education and Science of the Republic of Macedonia (project No. 16-11935/1).
Uncontrolled Keywords:	lipochrome ; melanin ; hemosiderin ; macrophage aggregates ; heavy metals
Subjects:	NATURAL SCIENCES > Biology
Divisions:	Division for Marine and Enviromental Research
Projects:	Project title Project leader Project code Project type Stanične promjene u vodnih organizama pobuđene metalima- Marijana Erk 098-0982934-2721 MZOS Patologija organizama iz voda u odnosu na zagađivala i akvakulturu- Damir Kapetanović 098-0982934-2752 MZOS
Depositing User:	Vlatka Filipović Marijić
Date Deposited:	11 Sep 2020 09:49
URI:	http://fulir.irb.hr/id/eprint/5938
DOI:	10.1002/jemt.23506

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