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Role of Sirt3 in Differential Sex-Related Responses to a High-Fat Diet in Mice

Pinterić, Marija; Iva, Škrinjar; Popović-Hadžija, Marijana; Tartaro Bujak, Ivana; Dekanić, Ana; Bagarić, Robert; Farkaš, Vladimir; Sobočanec, Sandra; Balog, Tihomir (2020) Role of Sirt3 in Differential Sex-Related Responses to a High-Fat Diet in Mice. Antioxidants, 9 (2). ISSN 2076-3921

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Metabolic homeostasis is differently regulated in males and females. Little is known about the mitochondrial Sirtuin 3 (Sirt3) protein in the context of sex-related differences in the development of metabolic dysregulation. To test our hypothesis that the role of Sirt3 in response to a high-fat diet (HFD) is sex-related, we measured metabolic, antioxidative, and mitochondrial parameters in the liver of Sirt3 wild-type (WT) and knockout (KO) mice of both sexes fed with a standard or HFD for ten weeks. We found that the combined effect of Sirt3 and an HFD was evident in more parameters in males (lipid content, glucose uptake, pparγ, cyp2e1, cyp4a14, Nrf2, MnSOD activity) than in females (protein damage and mitochondrial respiration), pointing towards a higher reliance of males on the effect of Sirt3 against HFD-induced metabolic dysregulation. The male-specific effects of an HFD also include reduced Sirt3 expression in WT and alleviated lipid accumulation and reduced glucose uptake in KO mice. In females, with a generally higher expression of genes involved in lipid homeostasis, either the HFD or Sirt3 depletion compromised mitochondrial respiration and increased protein oxidative damage. This work presents new insights into sex-related differences in the various physiological parameters with respect to nutritive excess and Sirt3.

Item Type: Article
Uncontrolled Keywords: sirtuin 3; high fat diet; sex differences; mice; oxidative stress; metabolic stress
NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology
Divisions: Division of Experimental Physics
Division of Materials Chemistry
Division of Molecular Medicine
Project titleProject leaderProject codeProject type
Sirtuin3 kao posrednik mitohondrijske funkcije u estrogen-ovisnoj otpornosti na oksidativni stres i prehranu s visokim udjelom masti-SuMERATihomir BalogIP-2014-09-4533HRZZ
Depositing User: Iva Škrinjar
Date Deposited: 10 Mar 2020 13:26
DOI: 10.3390/antiox9020174

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