Glutamic acid‐related hydrazine reagent for the derivatization of carbonyl compounds

Brkljačić, Lidija; Jerić, Ivanka (2018) Glutamic acid‐related hydrazine reagent for the derivatization of carbonyl compounds. Journal of Mass Spectrometry, 53 . pp. 649-654. ISSN 1076-5174

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Abstract

Chemical derivatization of carbonyl compounds is a prerequisite for their determination in environmental and biological samples by LC-MS/MS analysis. The main requirements in regard to the good derivatization reagent are fast, selective and quantitative reaction with carbonyl compounds, and efficient fragmentation upon collision-induced dissociation (CID). We have prepared glutamic acid-related hydrazine reagent, and optimized derivatization procedure with benzaldehyde as a model carbonyl compound. The CID spectrum of the obtained hydrazone gave rich fragmentation pattern with numerous abundant fragment ions. Structure-specific fragment ions were selected for the development of sensitive multiple reaction monitoring (MRM) method.

Item Type:	Article
Uncontrolled Keywords:	carbonyl compounds; derivatization; MS/MS analysis; hydrazine reagent; hydrazones
Subjects:	NATURAL SCIENCES > Chemistry > Analytic Chemistry
Divisions:	Division of Organic Chemistry and Biochemistry
Projects:	Project title Project leader Project code Project type Višekomponentne reakcije u sintezi peptidnih mimetika-MIMICRy IVANKA JERIĆ IP-2014-09-3102 HRZZ
Depositing User:	Ivanka Jerić
Date Deposited:	19 Nov 2019 12:55
URI:	http://fulir.irb.hr/id/eprint/5071
DOI:	10.1002/jms.4201

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