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Electrochemical Determination of Metallothioneins by the Modified Brdička Procedure as an Analytical Tool in Biomonitoring Studies

Mijošek, Tatjana; Erk, Marijana; Filipović Marijić, Vlatka; Krasnići, Nesrete; Dragun, Zrinka; Ivanković, Dušica (2018) Electrochemical Determination of Metallothioneins by the Modified Brdička Procedure as an Analytical Tool in Biomonitoring Studies. Croatica chemica acta, 91 . pp. 475-480. ISSN 0011-1643

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Abstract

Present study aims to improve the modified Brdička procedure of metallothionein (MT) quantification in a way to reduce the analysis duration. Therefore, scan rate of the voltammetric sweep was increased from 0.0052 V s–1 to 0.013 V s–1 by lowering the duration of one step potential from 0.5 s to 0.2 s, which resulted in 2.5 times faster voltammetric sweep. Research confirmed high accuracy and reliability of MT measurements, in both standard sample and samples of heat-treated cytosols of liver, gills and the intestine of European chub and liver of brown trout. The method was confirmed as fast and reliable electrochemical technique for quantification of MTs and this time- saving improvement is especially useful and applicable in different biomonitoring studies which require the analyses of numerous biological samples and high sample throughput.

Item Type: Article
Uncontrolled Keywords: metallothioneins ; differential pulse voltammetry ; modified scan rate ; fish tissues ; biomarkers ; biomonitoring
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Division for Marine and Enviromental Research
Projects:
Project titleProject leaderProject codeProject type
Akumulacija, unutarstanično mapiranje i učinci metala u tragovima u akvatičkih orbganizama-AQUAMAPMETMarijana ErkIP-2014-09-4255HRZZ
Depositing User: Sofija Konjević
Date Deposited: 09 Jul 2019 10:33
Last Modified: 05 Dec 2019 12:59
URI: http://fulir.irb.hr/id/eprint/4670
DOI: 10.5562/cca3444

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