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Cytotoxicity of gamma irradiated aflatoxin B1 and ochratoxin A

Domijan, Ana M.; Marjanović Čermak, Ana Marija; Vulić, Ana; Tartaro Bujak, Ivana; Pavičić, Ivan; Pleadin, Jelka; Markov, Ksenija; Mihaljević, Branka (2019) Cytotoxicity of gamma irradiated aflatoxin B1 and ochratoxin A. Journal of environmental science and health. Part B: pesticides, food contaminants, and agricultural wastes, 54 (3). pp. 155-162. ISSN 0360-1234

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Abstract

Toxicity of gamma irradiated mycotoxins aflatoxin B1 (AFB1) and ochratoxin A (OTA) was investigated in vitro. AFB1 and OTA stock solutions (50 mM, in methanol) were gamma irradiated (5 and 10 kGy) and non-irradiated and irradiated mycotoxins solutions were tested for cytotoxicity on Pk15, HepG2 and SH-SY5Y cell lines (MTT assay, 1–500 lM concentration range ; 24 h exposure). Degradation of mycotoxin molecules was examined by liquid chromatography tandem mass spectrometry (HPLC-MS/MS). AFB1 and OTA radiolytic products were less toxic than the parent mycotoxins to all of the tested cell lines. Gamma irradiation even at 5 kGy had effect on AFB1 and OTA molecules however, this effect was dependent on chemical structure of mycotoxin. Since gamma irradiation at low dose reduced initial level of both mycotoxins, and gamma irradiated mycotoxins had lower toxicity in comparison to non-irradiated mycotoxins, it can be concluded that gamma irradiation could be used as decontamination method.

Item Type: Article
Uncontrolled Keywords: cytotoxicity ; food safety ; gamma irradiation ; mycotoxins ; reduction
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Division of Materials Chemistry
Depositing User: Ivana Tartaro Bujak
Date Deposited: 08 Jul 2019 12:34
Last Modified: 08 Jul 2019 12:36
URI: http://fulir.irb.hr/id/eprint/4665
DOI: 10.1080/03601234.2018.1536578

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