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Genome-wide miRNA profiling reinforces the importance of miR-9 in human papillomavirus associated oral and oropharyngeal head and neck cancer

Božinović, Ksenija; Sabol, Ivan; Dediol, Emil; Milutin Gašperov, Nina; Manojlović, Spomenka; Vojtechova, Zuzana; Tachezy, Ruth; Grce, Magdalena (2019) Genome-wide miRNA profiling reinforces the importance of miR-9 in human papillomavirus associated oral and oropharyngeal head and neck cancer. Scientific Reports, 1 . pp. 1-13. ISSN 2045-2322

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Abstract

Head and neck cancer is the sixth most common malignancy worldwide, predominantly developing from squamous cell epithelia (HNSCC). The main HNSCC risk factors are tobacco, excessive alcohol use, and the presence of human papillomavirus (HPV). HPV positive (+) cancers are etiologically different from other HNSCC and often show better prognosis. The current knowledge regarding HNSCC miRNA profiles is still incomplete especially in the context of HPV+ cancer. Thus, we analyzed 61 freshly collected primary oral (OSCC) and oropharyngeal (OPSCC) SCC samples. HPV DNA and RNA was found in 21% cases. The Illumina whole-genome small- RNA profiling by next-generation sequencing was done on 22 samples and revealed 7 specific miRNAs to HPV+ OSCC, 77 to HPV+ OPSCC, and additional 3 shared with both ; 51 miRNAs were specific to HPV OPSCC, 62 to HPV OSCC, and 31 shared with both. The results for 9 miRNAs (miR-9, -21, -29a, -100, -106b, -143 and -145) were assessed by reverse transcription- quantitative polymerase chain reaction on the whole study population. Cell signaling pathway analysis revealed differences between HPV+ and HPV HNSCC. Our findings compared with literature data reveal extensive heterogeneity of miRNA deregulation with only several miRNAs consistently affected, and miR-9 being the most likely HPV related miRNA.

Item Type: Article
Uncontrolled Keywords: miRNA; HPV; HNSCC; OSCC; OPSCC
Subjects: BIOMEDICINE AND HEALTHCARE
BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences
BIOMEDICINE AND HEALTHCARE > Clinical Medical Sciences > Oncology
Divisions: Division of Molecular Medicine
Projects:
Project titleProject leaderProject codeProject type
Epigenetičke promjene karcinoma pločastih stanica glave i vrata-EpiC-HNSCCMagdalena GrceIP-2013-11-4758HRZZ
Depositing User: Ivan Sabol
Date Deposited: 27 May 2019 07:15
Last Modified: 27 May 2019 07:15
URI: http://fulir.irb.hr/id/eprint/4519
DOI: 10.1038/s41598-019-38797-z

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