Božinović, Ksenija; Sabol, Ivan; Dediol, Emil; Milutin Gašperov, Nina; Manojlović, Spomenka; Vojtechova, Zuzana; Tachezy, Ruth; Grce, Magdalena (2019) Genome-wide miRNA profiling reinforces the importance of miR-9 in human papillomavirus associated oral and oropharyngeal head and neck cancer. Scientific Reports, 9 . ISSN 2045-2322
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Abstract
Head and neck cancer is the sixth most common malignancy worldwide, predominantly developing from squamous cell epithelia (HNSCC). The main HNSCC risk factors are tobacco, excessive alcohol use, and the presence of human papillomavirus (HPV). HPV positive (+) cancers are etiologically different from other HNSCC and often show better prognosis. The current knowledge regarding HNSCC miRNA profiles is still incomplete especially in the context of HPV+ cancer. Thus, we analyzed 61 freshly collected primary oral (OSCC) and oropharyngeal (OPSCC) SCC samples. HPV DNA and RNA was found in 21% cases. The Illumina whole-genome small- RNA profiling by next-generation sequencing was done on 22 samples and revealed 7 specific miRNAs to HPV+ OSCC, 77 to HPV+ OPSCC, and additional 3 shared with both ; 51 miRNAs were specific to HPV OPSCC, 62 to HPV OSCC, and 31 shared with both. The results for 9 miRNAs (miR-9, -21, -29a, -100, -106b, -143 and -145) were assessed by reverse transcription- quantitative polymerase chain reaction on the whole study population. Cell signaling pathway analysis revealed differences between HPV+ and HPV HNSCC. Our findings compared with literature data reveal extensive heterogeneity of miRNA deregulation with only several miRNAs consistently affected, and miR-9 being the most likely HPV related miRNA.
| Item Type: | Article | ||||||||
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| Uncontrolled Keywords: | miRNA ; HPV ; HNSCC ; OSCC ; OPSCC | ||||||||
| Subjects: | BIOMEDICINE AND HEALTHCARE BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences BIOMEDICINE AND HEALTHCARE > Clinical Medical Sciences > Oncology |
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| Divisions: | Division of Molecular Medicine | ||||||||
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| Depositing User: | Ivan Sabol | ||||||||
| Date Deposited: | 27 May 2019 07:15 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/4519 | ||||||||
| DOI: | 10.1038/s41598-019-38797-z |
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