hrvatski jezikClear Cookie - decide language by browser settings

Live cell X-ray imaging of autophagic vacuoles formation and chromatin dynamics in fission yeast

Strelnikova, Natalja; Sauter, Nora; Guizar- Sicairos, Manuel; Göllner, Michael; Diaz, Ana; Delivani, Petrina; Chacon, Mariola R.; Tolić, Iva M.; Zaburdaev, Vasily; Pfohl, Thomas (2017) Live cell X-ray imaging of autophagic vacuoles formation and chromatin dynamics in fission yeast. Scientific Reports, 2017 (7). ISSN 2045-2322

PDF - Published Version - article
Available under License Creative Commons Attribution.

Download (4MB) | Preview


Seeing physiological processes at the nanoscale in living organisms without labeling is an ultimate goal in life sciences. Using X-ray ptychography, we explored in situ the dynamics of unstained, living fission yeast Schizosaccharomyces pombe cells in natural, aqueous environment at the nanoscale. In contrast to previous X-ray imaging studies on biological matter, in this work the eukaryotic cells were alive even after several ptychographic X-ray scans, which allowed us to visualize the chromatin motion as well as the autophagic cell death induced by the ionizing radiation. The accumulated radiation of the sequential scans allowed for the determination of a characteristic dose of autophagic vacuole formation and the lethal dose for fission yeast. The presented results demonstrate a practical method that opens another way of looking at living biological specimens and processes in a time-resolved label-free setting.

Item Type: Article
Uncontrolled Keywords: biological physics ; nanoscale biophysics ; X-rays
Subjects: NATURAL SCIENCES > Physics
Divisions: Division of Molecular Biology
Depositing User: Iva Tolić
Date Deposited: 04 Mar 2019 16:15
DOI: 10.1038/s41598-017-13175-9

Actions (login required)

View Item View Item


Downloads per month over past year

Increase Font
Decrease Font
Dyslexic Font