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A novel ABC transporter: the first insight into zebrafish (Danio rerio) Abch1

Popović, Marta; Žaja, Roko; Lončar, Jovica; Smital, Tvrtko (2010) A novel ABC transporter: the first insight into zebrafish (Danio rerio) Abch1. Marine Environmental Research, 69 (S1). S11-S13. ISSN 0141-1136

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Abch1 is a novel ABC transporter found in fish lineage. The aim of this study was to characterise zebrafish Abch1 using phylogenetic analysis, membrane topology prediction and tissue expression pattern determined by qRT-PCR. Phylogenetic analysis confirmed that no Abch1 orthologs can be found in other vertebrates, but similar genes have been found in invertebrate species. Abch1 is most closely related to the ABCG subfamily, although it shares only 12-14% of amino acid sequence identity with ABCG subfamily members. Topology analysis indicated that Abch1 is a half transporter that consists of six transmembrane domains with reverse domain arrangement (TMD-ABC), like ABCG subfamily members, but with differences in loop organization. Tissue distribution pattern revealed the highest Abch1 expression in brain and gill, followed by lower expression in gut, gonads and liver. Taken together, we hypothesise that fish Abch1 may be either involved in sterol transport similar to ABCG1, or is a part of the MXR defence like ABCG2. These questions remain to be addressed in further research.

Item Type: Article
Uncontrolled Keywords: Abch subfamily ; zebrafish ; phylogeny ; membrane topology ; tissue distribution
Subjects: NATURAL SCIENCES > Biology
NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology
Divisions: Division for Marine and Enviromental Research
Project titleProject leaderProject codeProject type
Ekotoksikološko značenje ABC transportnih proteina u vodenih organizama-Tvrtko Smital098-0982934-2745MZOS
Depositing User: Marta Popović
Date Deposited: 07 Nov 2018 14:50
DOI: 10.1016/j.marenvres.2009.10.016

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