Purification of enzymatically inactive peptidylarginine deiminase type 6 from mouse ovary that reveals hexameric structure different from other dimeric isoforms

Taki, Hirofumi; Gomi, Tomoharu; Knuckley, Bryan; Thompson, R Paul; Vugrek, Oliver; Hirata, Kazuya; Miyahara, Tatsurou; Shinoda, Kouichiro; Hounoki, Hiroyuki; Sugiyama, Eiji; Usui, Isao; Urakaze, Masaharu; Tobe, Kazuyuki; Ishimoto, Tetsuya; Inoue, Ran: Tanaka; Mano, Hiroki; Ogawa, Hirofumi; Mori, Hisashi (2011) Purification of enzymatically inactive peptidylarginine deiminase type 6 from mouse ovary that reveals hexameric structure different from other dimeric isoforms. Advances in Bioscience and Biotechnology, 2 . pp. 304-310. ISSN 2156-8456

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Abstract

The murine peptidylarginine deiminase (PAD) has five isoforms encoded by different genes and partici- pates in a variety of cellular functions through the citrullination of target proteins. The crystal structure of human PAD4 with a dimeric form was previously solved because of the enzyme’s relevance to rheuma- toid arthritis. PAD6, abundant in mouse oocytes and eggs, is believed to take part in early events of embryogenesis, but its biochemical properties are little understood. Here we have purified and charac- terized a recombinant PAD6. A PAD6 cDNA was cloned from mouse ovary RNA and expressed in Escherichia coli through pET29 and pGEX vectors. When benzoyl-L-arginine ethyl ester was used as a substrate, no appreciable activity was detected with a cell homogenate under conditions where a human PAD4 cDNA caused significant activity. Both pro- teins were affinity-purified to near homogeneity. The circular dichroism spectra of PAD6 and human PAD4 were similar in the far ultraviolet region. On molecular sieving, PAD6 was eluted faster than human PAD4. The cross-linking of PAD6 with dime- thyl suberimidate clearly showed six bands on an sodium dodecyl sulfate-polyacrylamide gel. These results indicate that PAD6 can constitute a hexameric structure. The purified PAD6 still showed no enzy- matic activity. This unique structure and loss in enzymatic activity is strongly suggested to favor the formation of egg cytoplasmic sheets as the architectu- ral protein.

Item Type:	Article
Uncontrolled Keywords:	Peptidylarginine Deiminase ; Isoform ; Dimer ; Hexamer ; Mouse Oocytes ; Cytoplasmic Sheets
Subjects:	NATURAL SCIENCES > Biology
Divisions:	Division of Molecular Medicine
Projects:	Project title Project leader Project code Project type Nedostatak Adenozilhomocistein hidrolaze: Molekularni mehanizmi novog oboljenja- Oliver Vugrek 098-0000000-2463 MZOS
Depositing User:	Oliver Vugrek
Date Deposited:	30 Aug 2018 10:52
URI:	http://fulir.irb.hr/id/eprint/4162
DOI:	10.4236/abb.2011.24044

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