Multilevel ecotoxicity assessment of environmentally relevant bisphenol A concentrations using the soil invertebrate Eisenia fetida

Babić, Sanja; Barišić, Josip; Bielen, Ana; Bošnjak, Ivana; Sauerborn Klobučar, Roberta; Ujević, Ivana; Strunjak-Perović, Ivančica; Topić Popović, Natalija; Čož-Rakovac, Rozelindra (2016) Multilevel ecotoxicity assessment of environmentally relevant bisphenol A concentrations using the soil invertebrate Eisenia fetida. Journal of Hazardous Materials, 318 . pp. 477-486. ISSN 0304-3894

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Abstract

Bisphenol A (BPA) presents a serious threat to soil ecosystems, yet its effects on soil-inhabiting organ-isms are mostly unexplored. Therefore, the impact of environmentally relevant BPA concentrations ona terrestrial model organism, the earthworm Eisenia fetida, was assessed. Animals were cutaneously exposed to 100 nM and 10 uM BPA up to 10 days (10-d). Next, a battery of biomarkers was used forecotoxicological evaluation on a cellular, tissue and behavioural level. HPLC analysis showed that aftera 10-d exposure, BPA accumulation reached a maximum of 2.50 ug BPA per g of wet tissue weight. Onthe cellular level, up to 3-d BPA exposure caused increased lipid oxidation indicating oxidative stress. Histopathological assessment of cell wall and ovaries after 7- and 10-d BPA exposure showed multipleabnormalities, i.e. hyperplasia of epidermis, increased body wall thickness and ovarian atrophy. Detec-tion of these changes was facilitated by a newly proposed semi-quantitative scoring system. Finally, behavioural changes were detected after only 3 days of exposure to 100 nM BPA. Altogether, the presented multilevel toxicity evaluation indicates high sensitivity of earthworms to low BPA doses.

Item Type:	Article
Additional Information:	This work has been supported by Ministry of Science, Education and Sport, Republic of Croatia (Project No. 098-1782739-2749) and Croatian Science Foundation (Project No. BIOTA UIP-11-2013-6433).
Uncontrolled Keywords:	bisphenol A; eisenia fetida; filter paper contact tes; oxidative stress; histopathology
Subjects:	NATURAL SCIENCES NATURAL SCIENCES > Biology NATURAL SCIENCES > Biology > Ecology NATURAL SCIENCES > Interdisciplinary Natural Sciences NATURAL SCIENCES > Interdisciplinary Natural Sciences > Environmental Science INTERDISCIPLINARY AREAS OF KNOWLEDGE
Divisions:	Division of Materials Chemistry
Projects:	Project title Project leader Project code Project type Substanična biokemijska i filogenetska raznolikost tkiva riba, rakova i školjaka- Rozelinda Čož-Rakovac 098-1782739-2749 MZOS Bioindikatori vodenih masa u Jadranu-BIOTA Zrinka Ljubešić UIP-2013-11-6433 HRZZ
Depositing User:	Sanja Babić
Date Deposited:	07 Dec 2017 10:35
URI:	http://fulir.irb.hr/id/eprint/3754
DOI:	10.1016/j.jhazmat.2016.07.017

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