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Gas phase structure of protonated histidine and histidine methyl ester - a combined experimental mass spectrometry and theoretical ab initio study

Kovačević, Borislav; Rožman, Marko; Klasinc, Leo; Srzić, Dunja; Maksić, Zvonimir; Yánez, Manuel (2005) Gas phase structure of protonated histidine and histidine methyl ester - a combined experimental mass spectrometry and theoretical ab initio study. Journal of Physical Chemistry A, 109 . pp. 8329-8335. ISSN 1089-5639

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Abstract

Gas phase H/D exchange experiments with CD3OD and D2O and quantum chemical ab initio G3(MP2) calculations were carried out on protonated histidine and protonated histidine methyl ester in order to elucidate their bonding and structure. The H/D exchange experiments show that both ions have three equivalent fast and one appreciably slower exchangeable hydrogen(s) assigned to the protonated amino group participating in a strong intramolecular hydrogen bond (IHB) with the nearest N(sp2) nitrogen of the imidazole fragment and to the distal ring NH-group, respectively. It is taken for granted that the proton exchange in the IHB is much faster than the H/D exchange. Unlike in other protonated amino acids (glycine, proline, phenylalanine, tyrosine and tryptophan) studied earlier, the exchange rate of the carboxyl group in protonated histidine is slower than that of the amino group. The most stable conformers and the enthalpies of neutral and protonated histidine and its methyl ester are calculated at the G3(MP2) level of theory. It is shown that strong intramolecular hydrogen bonding between the amino group and the imidazole ring nitrogen sites is responsible for the stability and specific properties of the protonated histidine. It is found that proton fluctuates between the amino and imidazole groups in the protonated form across an almost vanishing barrier. Proton affinity (PA) of histidine calculated by the G3(MP2) method is 233.2 and 232.4 kcal mol-1 for protonation at the imidazole ring and at the amino group nitrogens, respectively, which is about 3-5 kcal mol-1 lower than the reported experimental value.

Item Type: Article
Uncontrolled Keywords: gas phase structure of protonated histidine; gas phase structure of histidine methyl ester
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Division of Physical Chemistry
Depositing User: Kristina Ciglar
Date Deposited: 20 Jan 2017 15:37
Last Modified: 20 Jan 2017 15:37
URI: http://fulir.irb.hr/id/eprint/3341
DOI: 10.1021/jp053288t

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