Genetic requirements for conjugational recombination in the presence of lambda Gam protein in Escherichia coli

Ivančić-Baće, Ivana; Škrobot Vidaček, Nikolina; Zahradka, Davor; Salaj-Šmic, Erika; Brčić-Kostić, Krunoslav (2002) Genetic requirements for conjugational recombination in the presence of lambda Gam protein in Escherichia coli. Food Technology and Biotechnology, 40 (4). pp. 261-265. ISSN 1330-9862

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Abstract

The Gam protein of phage λ is a well-known inhibitor of the enzymatic activities of the RecBCD enzyme, the major enzyme involved in homologous recombination in bacteria Escherichia coli. In this work, we studied (i) the effect of the RecA loading-deficient recB (recBD1080A) mutation on conjugational recombination in the presence of λ phage Gam protein and (ii) additional genetic requirements for the RecBCD Gam-mediated conjugational recombination. For this purpose, we introduced Gam+ and Gam– expressing plasmids into wild type cells and different mutants of E. coli (recJ, recBD1080A, recB, recN, recF, recR, recO, recD), and determined the yields of recombinants after Hfr mediated conjugation. The obtained results suggest that RecA loading activity is not inhibited by Gam and that conjugational recombination in the presence of Gam is partially dependent on recJ and recO gene products.

Item Type:	Article
Uncontrolled Keywords:	Escherichia coli; phage lambda; conjugational recombination; RecBCD enzyme; Gam protein
Subjects:	NATURAL SCIENCES > Biology
Divisions:	Division of Molecular Biology
Projects:	Project title Project leader Project code Project type Raznolikost , struktura i evolucija genoma viših biljaka i nekih životinja Dražena Papeš 0119112 UNSPECIFIED Regulacija rekombinacije i rekombinacijskog popravka Dr.sc. Mirjana Petranović 0098071 UNSPECIFIED Uloga rekombinacije u popravku DNA i stabilnosti genoma Erika Salaj-Šmic 0098070 UNSPECIFIED
Depositing User:	Sofija Konjević
Date Deposited:	11 Apr 2016 13:22
URI:	http://fulir.irb.hr/id/eprint/2702

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