LOH of PTCH1 region in BCC and ovarian carcinoma: microsatellite vs. HRM analysis

Musani, Vesna; Sabol, Maja; Car, Diana; Ozretić, Petar; Orešković, Slavko; Leović, Dinko; Levanat, Sonja (2012) LOH of PTCH1 region in BCC and ovarian carcinoma: microsatellite vs. HRM analysis. Frontiers in bioscience (Elite edition), E4 (3). pp. 1049-1057. ISSN 1945-0494

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Official URL: http://dx.doi.org/10.2741/440

Abstract

Loss of heterozygosity (LOH) of tumor suppressor genes is a frequent event in tumorigenesis. LOH is most often analyzed by microsatellite typing, but here we offer a fast and efficient method for simultaneous SNP genotyping and mutation scanning, which can also be used for LOH detection. High resolution melting (HRM) provides simple variant detection, and can be adopted for a wide range of applications. When a melting profile for a specific SNP is determined, the screening can be done without the need for sequencing, and only the melting profiles differing from the established melting profiles should be sequenced. LOH of PTCH1 gene is often found in a series of different tumors, for example basal cell carcinoma (BCC) and ovarian carcinoma (OC). In this study LOH was detected in 50% of BCC and 27.27% of OC, and the detection rates of microsatellite typing and HRM were comparable. Both methods depended only on the heterozygosity of the loci analyzed, but HRM offers an additional advantage of detection of all sequence variants in the gene of interest.

Item Type:

Article

Uncontrolled Keywords:

loss of heterozygosity; high resolution melting; PTCH1; basal cell carcinoma; ovarian carcinoma

Subjects:

BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences

Divisions:

Division of Molecular Medicine

Projects:

Project title	Project leader	Project code	Project type
Signal transduction in tumors: Hh-Gli interactions and therapeutic potential (Prijenos signala u tumorima: Hh-Gli put, interakcije i potencijalne terapije)-	Sonja Levanat	098-0982464-2461	MZOS
National model of a hospital database for maxillofacial oncology (Nacionalni model kliničke baze podataka u maksilofacijalnoj onkologiji)-	Mihajlo Virag	108-1080057-0043	MZOS

Depositing User:

Maja Sabol

Date Deposited:

25 Nov 2015 11:27

URI:

http://fulir.irb.hr/id/eprint/2323

DOI:

10.2741/440