Carbohydrate-lectin interaction on graphene-coated surface plasmon resonance (SPR) interfaces

Penezić, Abra; Deokar, Geetanjali; Vignaud, Dominique; Pichonat, Emmanuelle; Subramanian, Palaniappan; Gašparović, Blaženka; Boukherroub, Rabah; Szunerits, Sabine (2014) Carbohydrate-lectin interaction on graphene-coated surface plasmon resonance (SPR) interfaces. Plasmonics, 9 (3). pp. 677-683. ISSN 1557-1955

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Abstract

The paper describes the detection of carbohydrate-lectin interaction on graphene-on-metal surface plasmon resonance (SPR) interfaces. Graphene-coated gold-based SPR interfaces were formed through the transfer of large-area graphene grown by chemical vapour deposition (CVD) on polycrystalline Cu foils. The method allowed successful transfer of single and double layered graphene sheets onto the SPR interfaces in a reproducible manner. Functionalization of the graphene interface with mannose was achieved by simple immersion into a mannose aqueous solution (100 mM), resulting in noncovalent -stacking interactions between the aromatic ring structure of graphene and mannose. The utility of the carbohydrate-modified graphene-on-gold interface for the selective and sensitive detection of carbohydrate-lectin interactions was demonstrated using model lectins from Lens culinaris (LC) and Triticum Vulgaris (TV). While LC lectin binds specifically to mannopyranoside units, TV lectin has an affinity for N-acetyl glucosamine and sialic acid residues.

Item Type:	Article
Uncontrolled Keywords:	graphene; surface plasmon resonance; mannose; lectins; biosensors
Subjects:	NATURAL SCIENCES > Chemistry NATURAL SCIENCES > Chemistry > Analytic Chemistry
Divisions:	Division for Marine and Enviromental Research
Projects:	Project title Project leader Project code Project type Nature of organic matter, interaction with traces and surfaces in environment (Priroda organske tvari, interakcije s mikrokonstituentima i površinama u okolišu) Irena Ciglenečki-Jušić 098-0982934-2717 MZOS
Depositing User:	Abra Penezić
Date Deposited:	20 Dec 2016 13:56
URI:	http://fulir.irb.hr/id/eprint/2209
DOI:	10.1007/s11468-014-9686-3

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