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Telomere Q-PNA-FISH - Reliable Results from Stochastic Signals

Ćukušić Kalajžić, Andrea; Škrobot Vidaček, Nikolina; Huzak, Miljenko; Ivanković, Milena; Rubelj, Ivica (2014) Telomere Q-PNA-FISH - Reliable Results from Stochastic Signals. PLoS ONE, 9 (3). e92559/1-e92559/10. ISSN 1932-6203

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Abstract

Structural and functional analysis of telomeres is very important for understanding basic biological functions such as genome stability, cell growth control, senescence and aging. Recently, serious concerns have been raised regarding the reliability of current telomere measurement methods such as Southern blot and quantitative polymerase chain reaction. Since telomere length is associated with age related pathologies, including cardiovascular disease and cancer, both at the individual and population level, accurate interpretation of measured results is a necessity. The telomere Q-PNA-FISH technique has been widely used in these studies as well as in commercial analysis for the general population. A hallmark of telomere Q-PNA-FISH is the wide variation among telomere signals which has a major impact on obtained results. In the present study we introduce a specific mathematical and statistical analysis of sister telomere signals during cell culture senescence which enabled us to identify high regularity in their variations. This phenomenon explains the reproducibility of results observed in numerous telomere studies when the Q-PNA-FISH technique is used. In addition, we discuss the molecular mechanisms which probably underlie the observed telomere behavior.

Item Type: Article
Uncontrolled Keywords: Telomeres; Q-PNA-FISH
Subjects: NATURAL SCIENCES > Mathematics
NATURAL SCIENCES > Biology
Divisions: Division of Molecular Biology
Projects:
Project titleProject leaderProject codeProject type
Struktura i funkcija telomera u kontroli staničnog rasta[108443] Ivica Rubelj098-0982913-2768MZOS
Statistička analiza slučajnih modela i primjene[168846] Miljenko Huzak037-0372790-2800MZOS
Depositing User: Ivica Rubelj
Date Deposited: 09 Jul 2015 08:06
Last Modified: 09 Jul 2015 08:06
URI: http://fulir.irb.hr/id/eprint/2040
DOI: 10.1371/journal.pone.0092559

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