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Influence of stress hormones on the auxin homeostasis in Brassica rapa seedlings

Salopek Sondi, Branka; Šamec, Dunja; Mihaljević, Snježana; Smolko, Ana; Pavlović, Iva; Janković, Iva; Ludwig-Müller, Jutta (2013) Influence of stress hormones on the auxin homeostasis in Brassica rapa seedlings. Plant Cell Reports, 32 (7). pp. 1031-1042. ISSN 0721-7714

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Abstract

The influence of stress hormones: jasmonic acid (JA), salicylic acid (SA), and abscisic acid (ABA) on one-day-old seedlings of Chinese cabbage (Brassica rapa L. ssp. pekinensis) was investigated with particular focus on auxin levels and the regulation of reversible auxin conjugation as a mechanism of auxin homeostasis. At the physiological level stress hormones inhibited root growth, where JA was the most prominent inhibitor with an IC50 value 3.1 μM, which is one and two orders of magnitude lower than that found for ABA and SA, respectively. JA treatment significantly increased the total auxin content, by induction of free and conjugated forms. Also, the stress hormones affected the transcription of genes involved in the process of the reversible auxin conjugation: auxin amidohydrolases BrIAR3 and BrILL2, and auxin conjugate synthetases BrGH3.1 and BrGH3.9. JA treatment increased the transcript level of BrIAR3 twofold, while it did not affect the transcription of BrILL2. SA and ABA down-regulated the transcription of both auxin amidohydrolase genes by 30%. Transcription of both auxin conjugate synthetases was significantly down-regulated by all treatments by 30-70%. Among the investigated biochemical stress markers, glutathione along with protein carbonylation appeared the most affected upon treatments. The redox status of the seedlings was shifted to the more oxidized state upon JA and ABA treatments, whereas SA caused more reduced redox state in comparison to the control. The principal component analysis (PCA) visualized relationship among auxin and stress parameters upon treatments. Accordingly, the role of auxin in stress response of Brassica seedlings was discussed.

Item Type: Article
Uncontrolled Keywords: auxin; jasmonic acid; abscisic acid; salicylic acid; reversible auxin conjugation; root growth inhibition
Subjects: NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology
NATURAL SCIENCES > Biology > Botany
Divisions: Division of Molecular Biology
Projects:
Project titleProject leaderProject codeProject type
Molekularna regulacija biljnog razvitka[200002] Branka Salopek-Sondi098-0982913-2829MZOS
Depositing User: Dunja Šamec
Date Deposited: 04 May 2015 09:23
Last Modified: 04 May 2015 09:23
URI: http://fulir.irb.hr/id/eprint/1805
DOI: 10.1007/s00299-013-1412-7

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