Antioxidant Properties of Extracts of Wild Medicinal Mushroom Species from Croatia

Piljac-Žegarac, Jasenka; Šamec, Dunja; Piljac, Ante; Mešić, Armin; Tkalčec, Zdenko (2011) Antioxidant Properties of Extracts of Wild Medicinal Mushroom Species from Croatia. International Journal of Medicinal Mushrooms, 13 (3). pp. 257-263. ISSN 1521-9437

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Abstract

Antioxidant activity and total phenol (TP) content of methanol and water extracts of three wild Croatian mushroom species Auricularia auricula-judae (Bull.) Quél., Sarcoscypha austriaca (Sacc.) Boud, and Strobilurus esculentus (Wulfen) Singer were determined and compared with the values obtained for extracts of four cultivated mushrooms Agaricus bisporus (J.E. Lange) Imbach (brown and white variant), Pleurotus ostreatus (Jacq.) P. Kumm. and Lentinula edodes (Berk.) Pegler. Spectrophotometric determination of the TP content was performed using the Folin-Ciocalteu method, while antioxidant activity was measured in a reaction with 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH assay) and ferric-tripyridyltriazine (Fe3+-TPTZ) complex (FRAP assay). On the average, 5.8-fold higher TP content was observed for water in comparison to methanol extracts of all analysed mushrooms. Consequently, antioxidant activity was also higher for water extracts, which is evident from the obtained higher values in the FRAP assay and lower EC50 values in the DPPH assay. Among the three tested wild species, the water extract of S. esculentus exhibited the highest concentration of TP, 8.12 mg/g gallic acid equivalents (GAE), the highest reducing power, 19.42 mmol Fe2+/kg, and the best radical scavenging properties, EC50 = 13.5 mg/mL.

Item Type:	Article
Uncontrolled Keywords:	Auricularia auricula-judae (Bull.) Quél. ; Sarcoscypha austriaca (Sacc.) Boud ; Strobilurus esculentus (Wulfen) ; mushrooms ; total phenols ; antioxidant activity ; radical scavenging ; reducing power
Subjects:	BIOTECHNICAL SCIENCES > Agronomy > Phytomedicine BIOTECHNICAL SCIENCES > Food Technology > Nutrition
Divisions:	Division for Marine and Enviromental Research Division of Molecular Biology
Projects:	Project title Project leader Project code Project type Ekološko modeliranje za održivo upravljanje resursima [25590] Tarzan Legović 098-0982934-2719 MZOS Molekularna regulacija biljnog razvitka [200002] Branka Salopek-Sondi 098-0982913-2829 MZOS
Depositing User:	Dunja Šamec
Date Deposited:	04 May 2015 12:35
URI:	http://fulir.irb.hr/id/eprint/1801
DOI:	10.1615/IntJMedMushr.v13.i3.50

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