Visualisation of basal cell carcinoma by fluorescence diagnosis and independent component analysis

Kopriva, Ivica; Peršin, Antun; Zorc, Hrvoje; Pašić, Aida; Lipozenčić, Jasna; Kostović, Krešimir; Lončarić, Martin (2007) Visualisation of basal cell carcinoma by fluorescence diagnosis and independent component analysis. Photodiagnosis and Photodynamic Therapy, 4 (3). pp. 190-196. ISSN 1572-1000

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Abstract

Photodynamic detection (PDD) of skin tumours is based on the visualization of a fluorophores, with the ability to accumulate in tumour tissue, by the use of fluorescence imaging. Of particular importance is the application of δ -5-aminolaevulinic acid (ALA) that, through the process of biosynthesis causes formation of the protoporphyrin IX (PpIX). The PpIX has the ability of selective fluorescence after basal cell carcinoma (BCC) has been treated with ALA. Higher concentration of PpIX in tumour tissue compared to surrounding normal skin is the basis for PDD. Our contribution in this preliminary study is application of the independent component analysis (ICA) to extract the BCC spatial map, by processing fluorescent RGB image acquired under excitation with 405 nm light. Comparative performance analysis with other two widely used image processing methods: ratio imaging and optimal threshold based imaging, reveals that ICA produces BCC spatial map that is most consistent in term of diagnostic quality by both visual assessment and calculation of the BCC demarcation line. We believe this represents a solid basis for the design of a compact and low-cost multi-spectral fluorescence imaging system, capable for real time calculation of the skin tumour demarcation.

Item Type:	Article
Uncontrolled Keywords:	basal cell carcinoma; photodynamic detection; fluorescence imaging; 5-aminolevulinic acid; independent component analysis
Subjects:	TECHNICAL SCIENCES > Computing > Data Processing BIOMEDICINE AND HEALTHCARE > Clinical Medical Sciences
Divisions:	Division of Laser and Atomic Research and Development
Projects:	Project title Project leader Project code Project type Primarni kožni limfomi u Hrvatskoj [236502] Romana Čeović 214-2180056-0052 MZOS UNSPECIFIED UNSPECIFIED 0108166 MZOS Analiza višespektralih podataka [217905] Ivica Kopriva 098-0982903-2558 MZOS UNSPECIFIED UNSPECIFIED TP-05/0098-47 UNSPECIFIED
Depositing User:	Hrvoje Zorc
Date Deposited:	07 May 2015 12:36
URI:	http://fulir.irb.hr/id/eprint/1788
DOI:	10.1016/j.pdpdt.2007.03.004

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