Identification and characterization of cytochrome P450 family 4 in the mussel, Mytilus galloprovincialis

Ravlić, Sanda (2014) Identification and characterization of cytochrome P450 family 4 in the mussel, Mytilus galloprovincialis. Doctoral thesis, Sveučilište u Zagrebu, Prirodoslovno-matematički fakultet.

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Abstract

Cytochrome P450 enzymes (CYPs) are essential components of cellular detoxification system. In this study seven new cytochrome P450 gene clusters from bivalve mollusc Mytilus galloprovincialis from different collection sites were identified and characterized. The phylogenetic analysis demonstrated that the identified sequences were clustered within the CYP4 branch. The deduced amino acid sequences showed high conservation of the residues and domains essential to the function of the enzyme. Identified gene transcripts corresponded to seven different gene clusters, each comprising a number of allelic variants, which were designated CYP4Y1, Y2, Y3, Y4, Y5, Y6 and Y7. Gene clusters CYP4Y2 and Y7, and CYP4Y5 and Y6 were site specific, while the gene clusters CYP4Y1, Y3 and Y4 were present at all investigated locations. Great expansion of the CYP4Y gene subfamily in the mussel M. galloprovincialis could be attributed to different environmental conditions. Data concerning diversity of CYP4Y genes could present a good base in further investigations for improvement of cytochrome P450 application as biomarker of marine environmental pollution.

Item Type:

Thesis (Doctoral thesis)

Uncontrolled Keywords:

cytochrome P450; cDNA cloning; phylogenetic analysis; environment, bivalvia

Subjects:

NATURAL SCIENCES
NATURAL SCIENCES > Interdisciplinary Natural Sciences
NATURAL SCIENCES > Interdisciplinary Natural Sciences > Marine Science

Divisions:

Center for Marine Research

Projects: