hrvatski jezikClear Cookie - decide language by browser settings

Deoxyribonucleases (DNases) in the cortex and endosome from marine sponge Tethya aurantium

Fafanđel, Maja; Ravlić, Sanda; Smodlaka Tanković, Mirta; Bihari, Nevenka (2010) Deoxyribonucleases (DNases) in the cortex and endosome from marine sponge Tethya aurantium. Russian Journal of Marine Biology, 36 (5). pp. 383-389. ISSN 1063-0740

[img] PDF - Accepted Version
Download (187kB)

Abstract

The presence and activity of deoxyribonucleases in the cortex and endosome sections from a sponge, the sea orange Tethya aurantium, were investigated. The maximal enzyme activity in sponge homogenate was detected at pH 4.27, pH 7.0 and pH 8.5–8.75. Among different specimens, several distinct patterns of neutral DNase isozymes were observed in the cortex section. In each investigated specimen the highest neutral DNase activity belonged to high molecular weight proteins (up to75 kDa). The acid DNases showed a low level of enzyme activity. In the endosome section the acid DNase activity was up to ten times higher than in the cortex and the presence of DNase II-like protein was detected. Neutral DNase, which expressed the highest enzyme activity in all the investigated specimens, has a molecular weight of 20 kDa and belongs to the DNase I-like family. The results indicate that the activity of neutral and acid DNases is related to sponge sections and their biological functions. The cortex, as the sponge section that communicates with the environment, expresses high interindividual variability and heterogeneity of neutral DNases, while the endosome section, where the intracellular digestion is localized, is a site of high acid DNase activity.

Item Type: Article
Uncontrolled Keywords: deoxyribonucleases, cortex, endosome; marine sponge; Tethya aurantium; acid DNase; neutral DNase
Subjects: NATURAL SCIENCES > Interdisciplinary Natural Sciences
Divisions: Center for Marine Research
Projects:
Project titleProject leaderProject codeProject type
Ekotoksični učinci onečišćenja na morske organizme[104652] Nevenka Bihari098-0982705-2725MZOS
Depositing User: Maja Fafanđel
Date Deposited: 29 Nov 2013 12:19
Last Modified: 07 Feb 2014 08:15
URI: http://fulir.irb.hr/id/eprint/1187
DOI: 10.1134/S1063074010050081

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year