Hemocytes/coelomocytes DNA content in five marine invertebrates: cell cycles and genome sizes

Fafanđel, Maja; Bihari, Nevenka; Smodlaka Tanković, Mirta; Ravlić, Sanda (2008) Hemocytes/coelomocytes DNA content in five marine invertebrates: cell cycles and genome sizes. Biologia, 63 (5). pp. 730-736. ISSN 0006-3088

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Abstract

The hemocytes/coelomocytes DNA content in five selected marine invertebrates (sea mouse Aphrodita aculeata, spiny crab Maja crispata, sea star Echinaster sepositus, sea urchin Paracentrotus lividus, tunicate Phallusia mammillata) was investigated by flow cytometry. The cell cycle analyses identified sea mouse coelomocytes as proliferating cells and revealed that spiny crab hemocytes and sea urchin coelomocytes complete their division in the hemolymph and coelom respectively. The genome sizes of sea mouse and spiny crab are reported for the first time. The diploid DNA content (2C) in sea mouse A. aculeata is 1.24 pg, of spiny crab M. crispata 7.46 pg, red starfish E. sepositus 1.52 pg and of sea urchin P. lividus 1.08 pg. The mean diploid DNA content in tunicate P. mamillata was 0.11 pg with high interindividual variability (45 %). The presented results provide a useful database for future studies in the field of invertebrate physiology, ecotoxicology, biodiversity, species conservation and phylogeny.

Item Type:	Article
Uncontrolled Keywords:	blood cells; flow cytometry; proliferation; genome size; cell cycle; invertebrates
Subjects:	NATURAL SCIENCES > Interdisciplinary Natural Sciences
Divisions:	Center for Marine Research
Projects:	Project title Project leader Project code Project type Ekotoksični učinci onečišćenja na morske organizme [104652] Nevenka Bihari 098-0982705-2725 MZOS
Depositing User:	Maja Fafanđel
Date Deposited:	29 Nov 2013 12:12
URI:	http://fulir.irb.hr/id/eprint/1186
DOI:	10.2478/s11756-008-0127-7

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