The Influence of a Protein Fragment Extracted from Abalone Shell Green Layer on the Precipitation of Calcium Carbonate Polymorphs in Aqueous Media

Njegić Džakula, Branka; Reggi, Michela; Falini, Giuseppe; Weber, Igor; Brečević, Ljerka; Kralj, Damir (2013) The Influence of a Protein Fragment Extracted from Abalone Shell Green Layer on the Precipitation of Calcium Carbonate Polymorphs in Aqueous Media. Croatica Chemica Acta, 86 (1). pp. 39-47. ISSN 0011-1643

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Abstract

Many living organisms form mineral phases through biologically controlled processes, known as biomineralization. Thus created materials are composites of both, mineral and organic components. The shell of the gastropod mollusc red abalone (Haliotis rufescens) consists of calcite and aragonite layers, each of them containing characteristic biopolymers responsible for biomineralization. In this work, the effect of interstitial green sheet polypeptide, GP, extracted from the green layer of the mollusc red abalone shell, on the process of spontaneous precipitation of calcium carbonate polymorphs, was investigated. Three precipitation systems, in which the initial mineralogical composition of the precipitate was different, have been studied. Thus, in system (1) calcite appeared, in system (2) a mixture of calcite and vaterite was found, while in system (3), ASW, only aragonite precipitated. However, the precipitation kinetic measurements, X-ray diffraction, FT-IR spectroscopy, and light and scanning electron microscopy indicated that the addition of GP in the model systems caused the inhibition of precipitation and change of morphology of crystals as a consequence of GP adsorption on the crystal surfaces and its entrapment into the mineral structure.

Item Type:	Article
Uncontrolled Keywords:	calcium carbonate; calcite; aragonite; vaterite; green sheet polypeptide (GP); crystal-growth; vaterite growth; biomphalaria-glabrata; precursor phase; kinetics; dissolution; biomineralization; crystallization; acid; morphologies
Subjects:	NATURAL SCIENCES > Chemistry
Divisions:	Division of Materials Chemistry Division of Molecular Biology
Projects:	Project title Project leader Project code Project type Regulacija dinamike citoskeleta u kretanju i diobi stanica [162634] Igor Weber 098-0982913-2858 MZOS Mehanizmi taloženja anorganskih biokompatibilnih i srodnih materijala [120470] Damir Kralj 098-0982904-2951 MZOS
Depositing User:	Branka Njegić Džakula
Date Deposited:	29 Nov 2013 09:52
URI:	http://fulir.irb.hr/id/eprint/1181
DOI:	10.5562/cca2197

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