Cytogenetic effects of Met-enkephalin (Peptid-M) on human lymphocytes

Štambuk, Nikola; Kopjar, Nevenka; Šentija, Karmela; Garaj-Vrhovac, Vera; Vikić-Topić, Dražen; Marušić-Della Marina, Branka; Brinar, Vesna; Trbojević-Čepe, Milica; Žarković, Neven; Ćurković, Božidar; Babić-Naglić, Đurđa; Hadžija, Mirko; Zurak, Niko; Brzović, Zdravko; Martinić, Roko; Štambuk, Vjera; Konjevoda, Paško; Ugrinović, Nikola; Pavlić-Renar, Ivana; Biđin, Zdenko; Pokrić, Biserka (1998) Cytogenetic effects of Met-enkephalin (Peptid-M) on human lymphocytes. Croatica Chemica Acta, 71 (3). pp. 591-605. ISSN 0011-1643

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Abstract

The structure, complementary structure and cytogenetic/proliferative effects of the Met-enkephalin on human peripheral blood lymphocytes were analyzed. Met-enkephalin, i.e. Peptid-M (LUPEX(R)), is a low molecular weight synthetic pentapeptide that corresponds to thymus Met-enkephalin, Its structure was examined by means of NMR spectroscopy. The influence of Met-enkephalin on in vitro normalization of chromosomally aberrant lymphocytes of patients suffering from different immune-mediated diseases, was analyzed by the sensitive cytogenetic tests for screening and detection of genome damages in human lymphocytes. The tests showed that in vitro stimulation of human lymphocytes with the Met-enkephalin led to disappearance of different types of chromosome aberrations, reduction in the number of micronuclei, decrease in the frequency of sister chromatid exchange (SCE) and apoptosis as well as a cytostatic effect on mitosis cycles. They have also confirmed normalization of chromosomally aberrant cell findings in patients suffering from different immune-mediated diseases. These results suggest a possible role of Met-enkephalin (Peptid-M) in immunotherapy of different diseases which involve chromosomal aberrations as well as abnormal cell proliferation and offer new approaches to immunotherapy by the use of Peptid-M. Based on the molecular recognition theory and the SCA method, peptide complementary to Peptid-M was designed, synthesized and denoted Peptide-D. Peptide-D isa calpastatin fragment. Predicted ligand-receptor interaction between both peptides is confirmed by the results showing that Peptide-D blocked the Peptid-M induced lymphocyte proliferation in a dose-dependent manner.

Item Type:	Article
Uncontrolled Keywords:	inhibition; kinetics; receptor; cytokine; protein; calpain; rat; DNA
Subjects:	NATURAL SCIENCES > Chemistry
Divisions:	Division of Molecular Medicine NMR Center
Projects:	Project title Project leader Project code Project type Ekogenetičko istraživanje djelovanja mutagena Verica Garaj-Vrhovac 00220107 MZOS Izotopno obilježavanje i molekulske spektroskopije Goran Baranović 00980802 MZOS Cijepljenje virusnim podjedinicama Biserka Pokrić 00981108 MZOS Uzroci i posljedice presađivanja endokrinog tkiva pankreasa Mirko Hadžija 00981109 MZOS Epizootiologija i suzbijanje zarazne bolesti burze Zdenko Biđin 053006 MZOS Oksidativni stres i bolesti središnjeg živčanog sustava Kamelija Žarković 108083 MZOS Apoptoza u oštećenjima središnjeg živčanog sustava Niko Zurak 108244 MZOS Oksidativni stres i zloćudne bolesti Neven Žarković 00981113 MZOS
Depositing User:	Nikola Štambuk
Date Deposited:	27 Nov 2013 10:42
URI:	http://fulir.irb.hr/id/eprint/1145

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