Protective effects of Met-enkephalin on alcohol induced gastric lesions

Konjevoda, Paško; Štambuk, Nikola; Vikić-Topić, Dražen; Boban-Blagaić, Alenka; Vikić-Topić, Smiljka; Mrljak, Vladimir; Ramadan, Pero; Biđin, Zdenko (2000) Protective effects of Met-enkephalin on alcohol induced gastric lesions. Croatica Chemica Acta, 73 (4). pp. 1111-1121. ISSN 0011-1643

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Abstract

The model of ethanol induced gastric lesions is useful in the evaluation of gastric cytoprotection. We used it to measure cytoprotective effects of two neuropeptides, Met-enkephalin (Peptid-M, LUPEX(R)) and alpha -melanocyte stimulating hormone (alpha -MSH). Both peptides exhibited significant and synergistic cytoprotective effects. The best therapeutic efficacy was obtained with Met-enkephalin and alpha -MSH mixture 3-5:1. Significant cytoprotective action on ethanol induced lesions was also observed by means of two thymus peptide immunomodulators, Thymus Peptide C(R) and JAN 50(R). Thymus Peptide C(R) exhibited a more significant synergistic effect with Met-enkephalin than JAN 50(R). In addition to the cytoprotection of rat gastric mucosa, Met-enkephalin also induced statistically significant and dose dependent Straub tail response versus control in mice. Haloperidol, naloxone and Peptide-D antagonised its effects. NMR spectrospic data supported molecular interaction of Metenkephalin and haloperidol, while neutralization of Met-enkephalin induced Straub tail response by means of Peptide-D indicated the presence of new non-opioid (naloxone independent) receptor system containing Peptide-D sequence (calpastatin 201-205 aa).

Item Type:	Article
Uncontrolled Keywords:	Met-enkephalin; LUPEX (R); alpha-MSH; ethanol; gastric lesions; peptide-D; cytoprotection; Straub phenomenon; haloperidol; NMR; Thymus Peptide C (R); JAN 50 (R); mucosal injury; alpha-msh; peptid-m; cyclooxygenase-2; neutrophils; rat
Subjects:	NATURAL SCIENCES > Chemistry
Divisions:	NMR Center
Projects:	Project title Project leader Project code Project type Izotopno obilježavanje i molekulske spektroskopije Goran Baranović 00980802 MZOS Cijepljenje virusnim podjedinicama Biserka Pokrić 00981108 MZOS Epizootiologija i suzbijanje zarazne bolesti burze Zdenko Biđin 053006 MZOS
Depositing User:	Nikola Štambuk
Date Deposited:	21 Nov 2013 12:56
URI:	http://fulir.irb.hr/id/eprint/1030

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